Mohn, Fabio, Weber, Michael, Schuebeler, Dirk and Roloff, Tim-Christoph (2009) Methylated DNA immunoprecipitation (MeDIP). Methods in Molecular Biology (Clifton, N.J.), 507. pp. 55-64. ISSN 1064-3745

Abstract

Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. In brief, genomic DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. The resulting enrichment of methylated DNA in the immunoprecipitated fraction can be determined by PCR to assess the methylation state of individual regions. Alternatively, MeDIP can be combined with large-scale analysis using microarrays as a genome-wide experimental readout. This protocol has been applied to generate comprehensive DNA methylation profiles on a genome-wide scale in mammals and plants, and further to identify abnormally methylated genes in cancer cells.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez...
Additional Information:	author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords:	MeDIP; methylated DNA; DNA methylation; genome-wide analysis; epigenomics; 5mC antibody; CpG; immunoprecipitation
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez...
Date Deposited:	14 Dec 2009 13:50
Last Modified:	31 Jan 2013 01:01
URI:	https://oak.novartis.com/id/eprint/988