Conformational flexibility of the dengue virus RNA dependent RNA polymerase revealed by a complex with an inhibitor
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Noble, Christian Guy, Lim, Siew Pheng, Chen, Yen Liang and Shi, Pei-Yong (2013) Conformational flexibility of the dengue virus RNA dependent RNA polymerase revealed by a complex with an inhibitor. Journal of Virology, 87 (9). pp. 5291-5295. ISSN 0022-538X
Abstract
We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype 3 (DENV-3), allowing structure refinement to a 1.79-Å resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor cocrystal structure at a 2.1-Å resolution. The inhibitor binds to the RdRp as a dimer and causes conformational changes in the protein. The improved crystallization conditions and new structural information should accelerate structure-based drug discovery.
| Item Type: | Article |
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| Date Deposited: | 13 Oct 2015 13:14 |
| Last Modified: | 13 Oct 2015 13:14 |
| URI: | https://oak.novartis.com/id/eprint/8739 |