Relationship of HIV-1 and SIV envelope glycoprotein trimer occupation and neutralization.
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Crooks, Emma T, Jiang, Pengfei, Franti, Michael, Wong, Sharon, Zwick, Michael B, Hoxie, James A, Robinson, James E, Moore, Penny L and Binley, James M (2008) Relationship of HIV-1 and SIV envelope glycoprotein trimer occupation and neutralization. Virology, 377 (2). pp. 364-378. ISSN 0042-6822
Abstract
Insights into the process of HIV-1 neutralization may assist rational vaccine design. Here, we compared antibody neutralization against the JR-FL primary isolate and trimer binding affinities judged by native PAGE. Monovalent Fab-trimer binding and neutralization showed a direct quantitative relationship, implying that neutralization begins as each trimer is occupied by one antibody. At saturation, three Fab or soluble CD4 molecules engaged each trimer. In contrast, a maximum of one soluble CD4 molecule bound to functional SIV trimers with a truncated a gp41 tail. Remarkably, soluble CD4 was found to trigger dramatic enhancement of this virus. Unlike Fabs, a quantitative correlation between JR-FL trimer binding and neutralization was unclear for some, but not all IgGs, as neutralization was markedly increased, but trimer affinity was largely unchanged. In addition, only one molecule of certain gp41-specific IgGs appeared to be able to bind each trimer. We discuss the implications of these findings in weighing the relative contributions of size, multivalent binding and other possible effects of IgGs to explain their increased potency.
| Item Type: | Article |
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| Additional Information: | author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used |
| Keywords: | HIV-1; SIV; Envelope; Glycoprotein; gp120; gp41; Antibody; Neutralization; Stoichiometry; Occupancy |
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| Date Deposited: | 14 Dec 2009 13:53 |
| Last Modified: | 14 Dec 2009 13:53 |
| URI: | https://oak.novartis.com/id/eprint/790 |