Influence of solvents and detergents on matrix-assisted laser desorption/ionization mass spectrometry measurements of proteins and oligonucleotides.
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Börnsen, K O, Gass, M A, Bruin, Gerardus, von Adrichem, J H, Biro, M C, Kresbach, G M and Ehrat, M (1997) Influence of solvents and detergents on matrix-assisted laser desorption/ionization mass spectrometry measurements of proteins and oligonucleotides. Rapid communications in mass spectrometry : RCM, 11 (6). pp. 603-609. ISSN 0951-4198
Abstract
The effect of solvents was found to be critical for sample preparation in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). For proteins and oligonucleotides the use of 2-propanol/water as a solvent for different matrices can significantly improve the quality of spectra. This effect is demonstrated with proteins ranging in molecular weight from 12 to 150 kDa and with a special 19-mer oligonucleotide. A comparison of MALDI-MS using of 2-propanol as matrix solvent and high-performance capillary electrophoresis resulted in identical relative peak intensities for a p(dT)12-18 oligonucleotide mixture. Additionally, the effect of detergents for characterization of high molecular weight proteins in very dilute solutions was studied with this solvent. It was found that Triton X-100, up to a concentration of 1%, was highly compatible with MALDI measurements and even could improve the quality of spectra. Use of detergents for cell profiling has extended the detectable mass range to about m/z 75,000.
| Item Type: | Article |
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| Date Deposited: | 31 Jan 2012 00:45 |
| Last Modified: | 01 Feb 2013 00:45 |
| URI: | https://oak.novartis.com/id/eprint/6537 |