Fritless capillary electrochromatography.
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Mayer, M, Rapp, E, Marck, C and Bruin, Gerardus (1999) Fritless capillary electrochromatography. Electrophoresis, 20 (1). pp. 43-49. ISSN 0173-0835
Abstract
The preparation of packed capillaries with stable frits of good quality can be a hurdle to obtain efficient separations in capillary electrochromatography (CEC). Especially with particles smaller than 3 microm, frit preparation is cumbersome. Highly efficient separations using packed capillaries without frits are presented. Under appropriate CEC conditions the particles were retained by electrophoretic attraction towards the anode by a tapered capillary inlet, without the need of a frit at the outlet end. Such fritless capillaries, packed with 1.5 microm nonporous reversed-phase particles, allowed separations with efficiencies of more than 500,000 plates/m. Once the capillaries were conditioned properly, more than 100 separations could be performed with good repeatability. With respect to separation efficiency, fritless capillaries packed with 3 microm particles were comparable with standard CEC capillaries with frits. Examples of separations of steroids, a pesticide and its by-products, and cardiac glycosides under various CEC conditions are shown.
| Item Type: | Article |
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| Date Deposited: | 31 Jan 2012 00:45 |
| Last Modified: | 01 Feb 2013 00:45 |
| URI: | https://oak.novartis.com/id/eprint/6475 |