Transient recombinant protein expression in a human amniocyte cell line: the CAP-T cell system
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Fischer, Simon, Charara, Nadine, Gerber, Andrea, Woelfel, Jens, Schiedner, Gudrun, Voedisch, Bernd and Geisse, Sabine (2012) Transient recombinant protein expression in a human amniocyte cell line: the CAP-T cell system. Biotechnology & Bioengineering, 109 (9). pp. 2250-2261. ISSN 0006-3592
Abstract
The impact of transient gene expression approaches (TGE) on the rapid production of recombinant proteins is undisputed, despite that all efforts are currently relying on two host cell families only, namely HEK293 derivatives and CHO cell line(s). Yet, the increasing complexity of biological targets calls for more than two host cell types to meet the challenges of difficult-to-express proteins. For this reason we evaluated the more recently established novel CAP-T® cell line derived from human amniocytes for its performance and potential in transient gene expression. Upon careful analyses and adaptation of all process parameters we show here that indeed the CAP-T® cells are extremely amenable to transient gene expression and recombinant protein production. Additionally, they possess inherent capabilities to express and secrete complex and difficult target molecules, thus adding an attractive alternative to the repertoire of existing host cell lines used in transient production processes.
| Item Type: | Article |
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| Additional Information: | author cannot archive publisher's version/PDF |
| Keywords: | CAP-T® cells, transient gene expression, transfection, polyethylenimine, lipofection, recombinant protein production |
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| Date Deposited: | 13 Oct 2015 13:14 |
| Last Modified: | 13 Oct 2015 13:14 |
| URI: | https://oak.novartis.com/id/eprint/6450 |