Bakhtiarova, Adel, Taslimi, Paul, Elliman, Stephen, Kosinski, Penelope, Hubbard, Brian, Kavana, Michael and Kemp, Daniel (2006) Resveratrol inhibits firefly luciferase. Biochemical and Biophysical Research Communications, 351 (2). pp. 481-484. ISSN 0006-291X

Abstract

The potential therapeutic value of resveratrol in age-related disease settings including cancer, diabetes, and Alzheimer's has emerged from a rapidly growing body of experimental evidence. Protection from oxidative stress appears to be a common feature of resveratrol that may be mediated through SirT1, though more specific molecular mechanisms by which resveratrol mediates its effects remain unclear. This has prompted an upsurge in cell-based mechanistic studies, often incorporating reporter assays for pathway elucidation in response to resveratrol treatment. Here, we report that resveratrol potently inhibits firefly luciferase with a K(i) value of 2microM, and caution that this confounding element may lead to compromised data interpretation.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... doi:10.1016/j.bbrc.2006.10.057
Additional Information:	author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords:	Resveratrol; Firefly luciferase; SirT1; Therapeutic; Drug; Cancer; Neurodegeneration
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... doi:10.1016/j.bbrc.2006.10.057
Date Deposited:	14 Dec 2009 13:57
Last Modified:	14 Dec 2009 13:57
URI:	https://oak.novartis.com/id/eprint/580