Wang, Yushi, Singer, Ryan, Liu, Xinyue, Inman, Seth J, Cao, Quynh, Zhou, Quan, Noble, Alex, Li, Laura, Arizpe Tafoya, Aidee Verónica, Babi, Mouhanad, Ask, Kjetil, Kolb, Martin R, Ramsay, Scott, Geng, Fei, Zhang, Boyang, Shargall, Yaron, Moran-Mirabal, Jose Manuel, Dabaghi, Mohammadhossein and Hirota, Jeremy A (2022) The CaT stretcher: An open-source system for delivering uniaxial strain to cells and tissues (CaT). Frontiers in bioengineering and biotechnology, 10. p. 959335. ISSN 2296-4185

Abstract

Integration of mechanical cues in conventional 2D or 3D cell culture platforms is an important consideration for and models of lung health and disease. Available commercial and published custom-made devices are frequently limited in breadth of applications, scalability, and customization. Herein we present a technical report on an open-source, cell and tissue (CaT) stretcher, with modularity for different and systems, that includes the following features: 1) Programmability for modeling different breathing patterns, 2) scalability to support low to high-throughput experimentation, and 3) modularity for submerged cell culture, organ-on-chips, hydrogels, and live tissues. The strategy for connecting the experimental cell or tissue samples to the stretching device were designed to ensure that traditional biomedical outcome measurements including, but not limited to microscopy, soluble mediator measurement, and gene and protein expression remained possible. Lastly, to increase the uptake of the device within

Item Type:	Article
Date Deposited:	28 Dec 2024 00:45
Last Modified:	28 Dec 2024 00:45
URI:	https://oak.novartis.com/id/eprint/56190