Allwood, Melissa A, Edgett, Brittany A, Platt, Mathew J, Marrow, Jade P, Coyle-Asbil, Bridget, Holjak, Emma J B, Nelson, Victoria L, Bangali, Swara, Alshamali, Razan, Jacyniak, Kathy, Klein, Jorden M, Farquharson, Laura, Romanova, Nadya, Northrup, Victoria, Ogilvie, Leslie M, Ayoub, Anmar, Ask, Kjetil, Vickaryous, Matthew K, Hare, Gregory M T, Brunt, Keith R and Simpson, Jeremy A (2024) Novel roles of cardiac-derived erythropoietin in cardiac development and function. Journal of molecular and cellular cardiology, 188. pp. 90-104. ISSN 1095-8584

Abstract

The role of erythropoietin (EPO) has extended beyond hematopoiesis to include cytoprotection, inotropy, and neurogenesis. Extra-renal EPO has been reported for multiple tissue/cell types, but the physiological relevance remains unknown. Although the EPO receptor is expressed by multiple cardiac cell types and human recombinant EPO increases contractility and confers cytoprotection against injury, whether the heart produces physiologically meaningful amounts of EPO in vivo is unclear. We show a distinct circadian rhythm of cardiac EPO mRNA expression in adult mice and increased mRNA expression during embryogenesis, suggesting physiological relevance to cardiac EPO production throughout life. We then generated constitutive, cardiomyocyte-specific EPO knockout mice driven by the Mlc2v promoter (EPOfl/fl:Mlc2v-cre+/-; EPO). During cardiogenesis, cardiac EPO mRNA expression and cellular proliferation were reduced in EPO hearts. However, in adult EPO mice, total heart weight was preserved through increased cardiomy

Item Type:	Article
Date Deposited:	28 Dec 2024 00:45
Last Modified:	28 Dec 2024 00:45
URI:	https://oak.novartis.com/id/eprint/56182