Pou Miralbell, Carla, Desrayaud, Sandrine, Oberhauser, Berndt, Reilly, John and Auberson, Yves (2025) Estimation of In Vivo Half-Life from In Vitro Metabolic Clearance, Protein and Cell Membrane Affinity Assays. ChemMedChem.

Abstract

The prediction of in vivo plasma half-life in rats from easily accessible in vitro assays is not possible yet. We surmised that the existing models, which consider protein binding and metabolic clearance, miss a term including the propensity to bind to membrane. We developed an improved proportionality equation taking all parameters into account. This article discusses when it can be applied and how reliable predictions are.

Item Type:	Article
Keywords:	half-life • model • CHI(IAM) • Human Serum Albumin • microsomal clearance
Date Deposited:	02 Sep 2025 00:46
Last Modified:	02 Sep 2025 00:46
URI:	https://oak.novartis.com/id/eprint/55652