Schober, Lukas, Dreier, Philippe and Peschke, Theo (2024) Automated high throughput workflow for rapid implementation of immobilized enzymes in chemical process development. Reaction Chemistry & Engineering. ISSN 2058-9883

Abstract

Despite known advantages of immobilized enzymes, their broad application in pharmaceutical drug manufacturing has not yet caught up. Limited access to commercially available immobilized enzymes on one side and time - and cost - consuming development of new processes on the other side have hampered their broader implementation so far. Herein, we present a walk-away high throughput workflow with state of the art robotic equipment that enables the rapid optimization and development of immobilized enzymes - ready to be scaled - and thus matching the need for speed in modern drug development. Following this workflow we were able to identify an engineered ketoreductase which was >2-fold more active than the wildtype from L. kefir. Further optimzation of the reaction and immobilization conditions lead to an additional 29-fold productivitiy increase from 0.36 to 10.6 gproduct/genzyme for the selected enzyme variant.

Item Type:	Article
Date Deposited:	06 Jun 2024 00:45
Last Modified:	06 Jun 2024 00:45
URI:	https://oak.novartis.com/id/eprint/52394