Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production.
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Geisse, Sabine and Henke, Mario (2005) Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production. Journal of Structural and Functional Genomics, 6 (2-3). pp. 165-170. ISSN 1345-711X
Abstract
Mammalian expression systems have an undisputed long-standing and very successful history for the generation of recombinant proteins, mainly as biopharmaceuticals. However, for use as 'tool proteins' in, e.g. assay development and screening, for structure elucidation and as antigens these expression systems were generally regarded as being cumbersome, tedious and expensive. This bias has largely been overcome with the very recent development of large-scale transient transfection (LST) approaches. Especially the HEK.EBNA expression system described here has contributed significantly to this success. The simplicity and speed of this approach compares well with expression trials using the widely applied Baculovirus/insect cell system. In addition, proteins generated in mammalian cells are usually correctly folded, fully processed and functionally active.
| Item Type: | Article |
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| Additional Information: | author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used |
| Keywords: | HEK.EBNA; mammalian cells; PEI; recombinant protein production; transient transfection |
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| Date Deposited: | 14 Dec 2009 13:59 |
| Last Modified: | 14 Dec 2009 13:59 |
| URI: | https://oak.novartis.com/id/eprint/497 |