Emperador-Melero, Javier, Wong, Man Yan, Wang, Shan Shan H, de Nola, Giovanni, Nyitrai, Hajnalka, Kirchhausen, Tom and Kaeser, Pascal S (2021) PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure. Nature communications, 12 (1). p. 3057. ISSN 2041-1723

Abstract

The active zone of a presynaptic nerve terminal defines sites for neurotransmitter release. Its protein machinery may be organized through liquid-liquid phase separation, a mechanism for the formation of membrane-less subcellular compartments. Here, we show that the active zone protein Liprin-α3 rapidly and reversibly undergoes phase separation in transfected HEK293T cells. Condensate formation is triggered by Liprin-α3 PKC-phosphorylation at serine-760, and RIM and Munc13 are co-recruited into membrane-attached condensates. Phospho-specific antibodies establish phosphorylation of Liprin-α3 serine-760 in transfected cells and mouse brain tissue. In primary hippocampal neurons of newly generated Liprin-α2/α3 double knockout mice, synaptic levels of RIM and Munc13 are reduced and the pool of releasable vesicles is decreased. Re-expression of Liprin-α3 restored these presynaptic defects, while mutating the Liprin-α3 phosphorylation site to abolish phase condensation prevented this rescue. Finally, PKC activation in these neurons acutely increased RIM, Munc13 and neurotransmitter release, which depended on the presence of phosphorylatable Liprin-α3. Our findings indicate that PKC-mediated phosphorylation of Liprin-α3 triggers its phase separation and modulates active zone structure and function.

Item Type:	Article
Date Deposited:	20 Jul 2021 00:45
Last Modified:	20 Jul 2021 00:45
URI:	https://oak.novartis.com/id/eprint/45307