Stumm, Michael, Walker, Maja Ruth, Stork-Fux, Caroline, Hanoteau, Noelle, O'Reilly, Terence and Wagner, Urs (2012) Validation of a post-fixation tissue storage and transport medium preserving histopathology and molecular pathology analyses (total and phospho-activated proteins, and FISH). American Journal of Clinical Pathology, 137 (3). pp. 429-436. ISSN 0002-9173

Abstract

Tumor biomarkers studies are integral to oncology clinical trials, but may yield artefactual results due to variation in sample procurement and processing. 70 % v/v ethanol was validated as a sample transport medium using markers of the PI3K/Akt/mTOR pathway. BT474 tumor xenografts were excised, slices immediately placed into formaldehyde and fixed for 24h. Fixed tissue slices were either immediately processed into paraffin or transferred to 70 % v/v ethanol and stored at room temperature for 1, 2, and 4 weeks before further processing. Freshly cut tissue sections were evaluated for pAKTS473, HER-2, pHER-2Y1248, pS6S235/236, and pS6S240/244, Ki-67, and FISH-HER2, and stained with H&E. No significant changes were observed when comparing samples stored in 70 % ethanol for up to 4 weeks with immediately processed tissue. 70 % v/v ethanol provides a safe storage medium for formaldehyde fixed tumor tissue facilitating sample transport during multi-center clinical trials.

Item Type:	Article
Additional Information:	author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Date Deposited:	13 Oct 2015 13:15
Last Modified:	13 Oct 2015 13:15
URI:	https://oak.novartis.com/id/eprint/4450