Barkley, Deborah, Hatsis, Panagiotis, Glick, Jim, Li, Wenkui and Flarakos, Jimmy (2020) Dixon's Q-test and Student's t-test to assess analog internal standard response in nonregulated LC-MS/MS bioanalysis. Bioanalysis, 12 (21). pp. 1535-1543. ISSN 17576199

Abstract

Aim: In bioanalytical assays, analyte response is normalized to an internal standard response. When the internal standard works well, it compensates for processing and detection variability. However, in case the internal standard introduces additional variability, due to addition errors or other issues, scientists need to identify this. Results: A new method, using a Q-test for outliers and a t-test to compare internal standard response from different sample types, is applied to 15 cases. The results show that the Q-test/t-test, which uses confidence level rather than arbitrary cut-points, is more discerning of deviations compared with widely used methods. Conclusion: This work may improve the quality of and rationale for the internal standard response monitoring method.

Item Type:	Article
Keywords:	analog internal standard Dixon’s Q-test internal standard response variation nonregulated LC–MS bioanalysis student’s t-test
Date Deposited:	03 Dec 2020 00:45
Last Modified:	03 Dec 2020 00:45
URI:	https://oak.novartis.com/id/eprint/43085