Auld, Douglas, Busby, Scott, Smith, Thomas, Renaud, Nicole, Carbonneau, Seth, Numao, Shin, Concannon, John and Dumelin, Christoph (2020) Advancements in assay technologies and strategies to enable drug discovery. ACS Chemical Biology. ISSN 1554-89291554-8937

Abstract

ABSTRACT: Assays drive drug discovery from the exploratory phases to the clinical testing of drug candidates. As such, numerous assay technologies and methodologies have arisen to support drug discovery efforts. Robust identification and characterization of tractable chemical matter requires biochemical, biophysical, and cellular approaches and often bene-fits from high-throughput methods. To increase throughput, efforts have been made to provide assays in miniaturized volumes which can be arrayed in microtiter plates to support the testing of as many as 100,000 samples/day. Alongside these efforts has been the growth of microtiter plate-free formats with encoded libraries that can support the screening billions of compounds, a hunt for new drug modalities, as well as the emphasis on more disease relevant formats using complex cell models of disease states. This review will focus on recent developments in high-throughput assay technolo-gies applied to identify starting points for drug discovery. We also provide recommendations on strategies for implement-ing various assay types to select high quality leads for drug development.

Item Type:	Article
Keywords:	high throughput screening, drug discovery, cell-based assays, biochemical assays, lead discovery
Date Deposited:	22 Oct 2020 00:45
Last Modified:	22 Oct 2020 00:45
URI:	https://oak.novartis.com/id/eprint/42877