Auld, Douglas (2020) Microplate Selection and Recommended Practices in High-throughput Screening and Quantitative Biology. Jun 2020.

Abstract

High-throughput screening (HTS) can efficiently efficiently assay multiple discrete biological reactions using multi-well microplates. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. This This chapter reviews key criteria for microplate selection, including: well number, well volume and shape, microplate color, surface treatments/coatings, and considerations for specialized applications such as high-content screening. This This chapter then provides important technical advice for microplate handling including mixing, dispensing, incubation, and centrifugation. Special topics are discussed, including microplate surface properties, plate washing, well-to-well contamination, microplate positional effects, effects,effects, well-to-well and inter-lot variability, and troubleshooting. This This information and best practices derived from academic, government, and industry screening centers, as well as microplate vendors, should accelerate assay development and enhance assay quality.

Item Type:	Article
Date Deposited:	30 Jun 2020 00:45
Last Modified:	30 Jun 2020 00:45
URI:	https://oak.novartis.com/id/eprint/42694