Growcott, Ellie, Coulthard, Alex, Amison, Richard, Hardaker, Liz, Saxena, Vishal, Malt, Layla, Jones, Peter, Grevot, Armelle, Poll, Christopher, Osborne, Colin and Banner, Kathy (2011) Characterisation of a Refined Rat Model of Respiratory Infection with Pseudomonas aeruginosa and the Effect of Ciprofloxacin. Journal of Cystic Fibrosis, 10 (3). pp. 166-174. ISSN 1569-1993

Abstract

BACKGROUND:

We sought to characterise a refined rat model of respiratory infection with P. aeruginosa over an acute time course and test the antibiotic ciprofloxacin.

METHODS:

Agar beads were prepared ± SPAN(®)80. Rats were inoculated with sterile agar beads or those containing 10(5) colony forming units (cfu) P. aeruginosa via intra-tracheal dosing. Bacterial load and inflammatory parameters were measured.

RESULTS:

Differing concentrations of SPAN(®) 80 modified median agar bead diameter and reduced particle size distribution. Beads prepared with 0.01% v/v SPAN(®)80 were evaluated in vivo. A stable lung infection up to 7 days post infection was achieved and induced BALF neutrophilia 2 and 5 days post infection. Ciprofloxacin (50mg/kg) significantly attenuated infection without affecting the inflammatory parameters measured.

CONCLUSION:

SPAN(®) 80 can control the particle size and lung distribution of agar beads and P. aeruginosa-embedded beads prepared with 0.01%v/v SPAN(®)80 can induce infection and inflammation over 7 days.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/pubmed/21247...
Related URLs:	http://www.ncbi.nlm.nih.gov/pubmed/21247...
Date Deposited:	13 Oct 2015 13:15
Last Modified:	13 Oct 2015 13:15
URI:	https://oak.novartis.com/id/eprint/4196