Identification of a Xist silencing domain 1 by Tiling CRISPR
Tools
Wang, Yang, Zhong, Yang, Zhou, Yingyao, Tanaseichuk, Olga, Wilkinson, Jeremy, Li, Zhizhong and Zhao, Jing Crystal (2019) Identification of a Xist silencing domain 1 by Tiling CRISPR. Scientific Reports.
Official URL: https://www.nature.com/articles/s41598-018-36750-0...
Abstract
Despite the essential roles of long noncoding RNAs (lncRNAs) in development and disease, methods determine lncRNA cis-elements are lacking. Here, we developed a screening method named “Tiling CRISPR” to identify lncRNA functional domains. Using this approach, we identified Xist A-Repeats as the silencing domain, an observation in agreement with published work. Mechanistic analysis suggested a novel function for Xist A-repeats in promoting Xist transcription.
Item Type: | Article |
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Keywords: | CRISPR, long noncoding RNA, Xist, A-Repeats, transcription |
Date Deposited: | 12 Mar 2019 00:45 |
Last Modified: | 12 Mar 2019 00:45 |
URI: | https://oak.novartis.com/id/eprint/36934 |