Laternser, Sandra, Keller, Hansjoerg, Leupin, Olivier, Rausch, Martin, Graf-Hausner, Ursula and Rimann, Markus (2018) A Novel Microplate 3D Bioprinting Platform for the Engineering of Muscle and Tendon Tissues. SLAS TECHNOLOGY: Translating Life Sciences Innovation. ISSN 2472-63032472-6311

Abstract

Two-dimensional (2D) cell cultures do not reflect the in vivo situation thus it is important to develop predictive 3D in vitro models with enhanced reliability and robustness for drug screening applications. Treatments against muscle-related diseases are becoming more prominent due to the growth of the aging population worldwide. Therefore, in this study, we describe a novel drug screening platform with automated production of 3D musculoskeletal-tendon-like tissues. With 3D bioprinting alternating layers of photo-polymerized gelatin-methacryloyl-based bioink and cells are deposited in a dumbbell shape onto novel post holder cell culture inserts in 24-well plates. Monocultures of human primary skeletal muscle cells and rat tenocytes were printed around and in between the posts that showed high viability and tissue differentiation in culture, based on marker gene and protein expressions. Different printing patterns of bioink and cells were explored and calcium signalling with Fluo4-loaded cells under electrical stimulation was shown. Finally, controlled co-printing of tenocytes and myoblasts around the posts and between the posts, respectively, was demonstrated followed by co-culture and co-differentiation.
This screening platform combining 3D bioprinting with a novel microplate represents a promising tool to address musculoskeletal diseases.

Item Type:	Article
Date Deposited:	03 Jul 2018 00:45
Last Modified:	03 Jul 2018 00:45
URI:	https://oak.novartis.com/id/eprint/35185