Abdubeck, Polat, Chen, Connie, Feuerhelm, Julie, Grant, Joanna, Bakolitsa, Constantina, Klock, Heath, Knuth, Mark, Mcmullan, Daniel, Okach, Linda, Puckett, Christina, Lesley, Scott and Stolz, Barbara (2010) GNF ID 100605: Crystal structure of BT_3984, the first representative of the SusD/RagB family of nutrient binding. Acta F.

Abstract

The crystal structure of Bacteroides thetaiotaomicron protein BT_3984 was solved to a resolution
of 1.7 Å by multiple-wavelength anomalous dispersion (MAD) using the semi-automated, highthroughput
pipeline of the Joint Center for Structural Genomics (JCSG), as part of the NIGMS
Protein Structure Initiative (PSI). BT_3984 is the first structure to be determined from the extensive
SusD family of polysaccharide binding proteins, which define a new paradigm for glycan utilization
in dominant members of the human gut microbiota. Structural analysis of BT_3984 reveals an Nterminal
region containing several tetratricopeptide repeats (TPR), while the signature C-terminal
region is less structured and contains extensive loop regions. Sequence and structure analysis of
BT_3984 suggests binding interfaces for other proteins from the polysaccharide utilization
complex

Item Type:	Article
Additional Information:	grant requirement B. Stolz is NOT an author -- NVS contact person for GNF manuscript approval. This manuscript shall NOT be copied to the OAK internet.
Keywords:	Structural Genomics, starch utilization system, gut microbiome, metagenomics
Date Deposited:	13 Oct 2015 13:16
Last Modified:	13 Oct 2015 13:16
URI:	https://oak.novartis.com/id/eprint/3112