Estoppey, David, Hewett, Jeffrey, Guy, Chantale, Harrington, Edmund, Thomas, Jason, Schirle, Markus, Cuttat-Theurillat, Rachel, Waldt, Annick, Gerrits, Bertran, Yang, Zinger, Schuierer, Sven, Carbone, Walter, Knehr, Judith, Lindeman, Alicia, Russ, Carsten, Frias, Elizabeth, Hoffman, Gregory, Varadarajan, Malini, Ramadan, Nadire, Reece-Hoyes, John, Wang, Qiong, Chen, Xin, Mcallister, Gregory, Roma, Guglielmo, Bouwmeester, Antonius and Hoepfner, Dominic (2017) Identification of a novel NAMPT inhibitor by CRISPR/Cas9 chemogenomic profiling in mammalian cells. Scientific reports, 7 (42728). pp. 1-6. ISSN 2045-2322

Abstract

Chemogenomic profiling is a powerful and unbiased approach to elucidate targets and mechanism of bioactive compounds. Until recently, high-quality experiments of this nature have been limited to fungal systems due to lack of mammalian genome-wide deletion collections. Here we show that the CRISPR/Cas9 system enables the generation of such libraries and allows for the identification of targets and pathways mediating hypersensitivity and resistance relevant to the compound mechanism of action, using a novel NAMPT inhibitor as an example.

Item Type:	Article
Date Deposited:	22 Feb 2017 00:45
Last Modified:	22 Feb 2017 00:45
URI:	https://oak.novartis.com/id/eprint/30845