Ebhardt, H. Alexander, Degen, Simone, Tadini, Valentina, Schilb, Alain, Greig, Carolyn A., Fearon, Kenneth CH, Aebersold, Ruedi and Jacobi, Carsten (2017) Comprehensive proteome analysis of muscle composition and development in seniors. EMBO Molecular Medicine.

Abstract

As cancer frequently occurs with old age, identifying and differentiating molecular mechanism mediating muscle wasting in cachectic and sarcopenic patient groups is sometimes challenging. We aimed to characterize proteome changes in human muscle under disease (cancer, cancer cachexia, sarcopenia) compared to healthy elderly patient cohort using LC-ESI mass spectrometry in SWATH-MS mode. We identified a potential protein signature which could be used to differentiate between sarcopenic and cachectic patient groups. To characterize this observation in more detail and mimic environmental challenges of muscle regeneration in elderly patients, we used an in vitro system of myogenesis. We investigated changes in the proteome under normal and with TNFα perturbed conditions as function of time. We identified alterations in key proteins of the energy metabolism and signaling pathway upon TNFα treatment, which reflect observed changes in the disease conditions. The work presented here lays the foundation for our understanding of muscle wasting diseases and holds the promise of defining a clinically relevant protein signature differentiating cachectic and sarcopenic patients.

Item Type:	Article
Date Deposited:	22 Mar 2017 00:45
Last Modified:	22 Mar 2017 00:45
URI:	https://oak.novartis.com/id/eprint/30504