Yap, Thai Leong, Chen, Yen Liang, Xu, Ting, Wen, Daying, Vasudevan, Subhash and Lescar, Julien (2007) A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 63 (Pt 2). pp. 78-83. ISSN 1744-3091

Abstract

Dengue virus, a member of the Flaviviridae genus, causes dengue fever, an important emerging disease with several million infections occurring annually for which no effective therapy exists. The viral RNA-dependent RNA polymerase NS5 plays an important role in virus replication and represents an interesting target for the development of specific antiviral compounds. Crystals that diffract to 1.85 A resolution that are suitable for three-dimensional structure determination and thus for a structure-based drug-design program have been obtained using a strategy that included expression screening of naturally occurring serotype variants of the protein, the addition of divalent metal ions and crystal dehydration.

Item Type:	Article
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Date Deposited:	14 Dec 2009 14:01
Last Modified:	31 Jan 2013 01:20
URI:	https://oak.novartis.com/id/eprint/305