A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution.
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Yap, Thai Leong, Chen, Yen Liang, Xu, Ting, Wen, Daying, Vasudevan, Subhash and Lescar, Julien (2007) A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 63 (Pt 2). pp. 78-83. ISSN 1744-3091
Abstract
Dengue virus, a member of the Flaviviridae genus, causes dengue fever, an important emerging disease with several million infections occurring annually for which no effective therapy exists. The viral RNA-dependent RNA polymerase NS5 plays an important role in virus replication and represents an interesting target for the development of specific antiviral compounds. Crystals that diffract to 1.85 A resolution that are suitable for three-dimensional structure determination and thus for a structure-based drug-design program have been obtained using a strategy that included expression screening of naturally occurring serotype variants of the protein, the addition of divalent metal ions and crystal dehydration.
| Item Type: | Article |
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| Additional Information: | free final full text version available at PubMedCentral; archiving not allowed on institutional repository |
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| Date Deposited: | 14 Dec 2009 14:01 |
| Last Modified: | 31 Jan 2013 01:20 |
| URI: | https://oak.novartis.com/id/eprint/305 |