Fernández-Golbano, Isabel Maria, Giblin, Jonathan, Idrissi, Fatima-Zahra, Grötsch, Helga, del Mar Borrás , Maria and Geli, Maria Isabel (2014) A cross-talk between PI(4,5)P2 and CK2´ modulates actin polymerization during endocytic uptake. Developmental Cell.

Abstract

A transient burst of actin polymerization assists membrane deformation and vesicle scission during endocytic budding but how actin polymerization is controlled in this context is not understood. Here, we show that a crosstalk between PI(4,5)P2 and one of the catalytic subunits of CK2 (Cka2) controls actin polymerization during endocytic budding. We find that phosphorylation of the myosin-I Myo5 by Cka2 down-regulates Arp2/3-dependent actin polymerization, whereas PI(4,5)P2 directly releases autoinhibition of the actin nucleating promoting activity of Myo5 and inhibits the catalytic activity of Cka2. Consistently, Cka2 and the PI(4,5)P2 5-phosphatases Sjl1 and Sjl2, the yeast synaptojanins, exhibit strong genetic interactions indicating functional redundancy. Our data demonstrate a novel holoenzyme-independent function of the yeast CK2α´ in endocytosis and establishes for the first time a strong genetic, functional and molecular link between two masters of intracellular signaling, PI(4,5)P2 and CK2.

Item Type:	Article
Date Deposited:	13 Oct 2015 13:12
Last Modified:	13 Oct 2015 13:12
URI:	https://oak.novartis.com/id/eprint/22864