He, Y, Wu, J, Mei, H, Yu, H, Sun, S, Shou, J and Li, H (2014) Histone deacetylase activity is required for embryonic posterior lateral line development. Cell Proliferation. pp. 91-104.

Abstract

Objectives: The posterior lateral line (PLL) system in zebrafish has recently become a model for investigating tissue morphogenesis. PLL primordium periodically deposits neuromasts as it migrates along the horizontal myoseptum from head to tail of the embryonic fish, and this migration requires activity of various molecular mechanisms. Histone deacetylases (HDACs) have been implicated in numerous biological processes of development, by regulating gene transcription, but their roles in regulating PLL during embryonic development have up to now remained unexplored. Material and methods: In this study, we used HDAC inhibitors to investigate the role of HDACs in early development of the zebrafish PLL sensory system. We further investigated development of the PLL by cell-specific immunostaining and in situ hybridization. Results: Our analysis showed that HDACs were involved in zebrafish PLL development as pharmacological inhibition of HDACs resulted in its defective formation. We observed that migration of PLL primordium was altered and accompanied by disrupted development of PLL neuromasts in HDAC inhibitor-treated embryos. In these, positions of PLL neuromasts were affected. In particular, the first PLL neuromast was displaced posteriorly in a treatment dose-dependent manner. Primordium cell proliferation was reduced upon HDAC inhibition. Finally, we showed that inhibition of HDAC function reduced numbers of hair cells in PLL neuromasts of HDAC inhibitor-treated embryos. Conclusion: Here, we have revealed a novel role for HDACs in orchestrating PLL morphogenesis. Our results suggest that HDAC activity is necessary for control of cell proliferation and migration of PLL primordium and hair cell differentiation during early stages of PLL development in zebrafish. 2013 John Wiley & Sons Ltd

Item Type:	Article
Additional Information:	NIBR author: Shou, J institute: NIBR contributor address: (He, Li) Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, China (He, Wu, Mei, Yu, Sun, Li) Department of Otolaryngology, Affiliated Eye and ENT Hospital of Fudan University, Shanghai 200031, China (Shou) China Novartis Institutes for BioMedical Research, Shanghai 201203, China (Li) State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200031, China
Date Deposited:	13 Oct 2015 13:12
Last Modified:	13 Oct 2015 13:12
URI:	https://oak.novartis.com/id/eprint/22589