Novel zwitterionic polyphosphorylcholine monolithic column for hydrophilic interaction chromatography.
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Jiang, Zhengjin, Reilly, John, Everatt, Brian and Smith, Norman W (2009) Novel zwitterionic polyphosphorylcholine monolithic column for hydrophilic interaction chromatography. Journal of Chromatography. A, 1216 (12). pp. 2439-2448. ISSN 1873-3778
Abstract
A novel porous zwitterionic monolith was prepared by thermal co-polymerisation of 2-methacryloyloxyethyl phosphorylcholine (MPC) and ethylene glycol dimethacrylate (EDMA) within 100 microm I.D. capillaries. Mercury intrusion porosimetry, scanning electron microscopy (SEM), micro-HPLC (micro-HPLC), elemental analysis and zeta-potential analysis were used to evaluate the monolithic structure. No evidence of swelling or shrinking of the monolith in different polarity solvents was observed. A typical hydrophilic liquid chromatography (HILIC) mechanism was observed at high organic solvent content (acetonitrile >60%). The phosphorylcholine (PC) functionality has both a positively charged quaternary ammonium and a negatively charged phosphate group. For charged analytes, a weak electrostatic interaction was also observed by studying the influence of mobile phase pH and salt concentration on their retentions on the poly(MPC-co-EDMA) monolithic column. The optimised poly(MPC-co-EDMA) monolith showed very good selectivities for a range of polar test analytes, especially small peptides. This might be ascribed to the good biocompatibility of PC functionality. At low organic solvent content, baseline separation was also observed for a test mixture of seven alkylphenones by a reversed-phase separation mechanism.
| Item Type: | Article |
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| Additional Information: | author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used |
| Keywords: | Capillary liquid chromatography; Monolith; Hydrophilic interaction chromatography; Phosphorylcholine; Weak electrostatic interaction; Zwitterionic |
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| Date Deposited: | 14 Dec 2009 13:48 |
| Last Modified: | 31 Jan 2013 00:54 |
| URI: | https://oak.novartis.com/id/eprint/1482 |