Ceramide Kinase Profiling by Mass Spectrometry reveals a Conserved Phosphorylation Pattern downstream of the Catalytic Site
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Chen, Wei-qiang, Graf, Christine, Zimmel, David, Rovina, Philipp, Krapfenbauer, Kurt, Jaritz, Markus, Parker, Peter, Lubec, Gert and Bornancin, Frederic (2009) Ceramide Kinase Profiling by Mass Spectrometry reveals a Conserved Phosphorylation Pattern downstream of the Catalytic Site. Journal of Proteome Research, 9 (1). pp. 420-429. ISSN 1535-3893
Abstract
Ceramide kinase (CERK) is essential for production of ceramide-1-phosphate (C1P), a bioactive lipid whose formation critically modulates ceramide levels. To explore how CERK is regulated we used insect cell-expressed, recombinant hCERK, and searched for post-translational modifications, using mass-spectrometry techniques. This led to identification of two phosphorylated serine residues, at positions 340 and 408. Point mutations preventing phosphorylation at either of these sites did not lead to detectable changes in subcellular localization or activity. However, preventing phosphorylation at S340 resulted in CERK instability as revealed by the behavior of the S340A mutant protein under various assay conditions in vitro. Phosphorylation of a cognate serine residue in sphingosine kinases was previously shown to be important. Therefore, phosphorylation within a conserved “regulation loop” downstream of the catalytic domain emerges as a new paradigm for regulation of kinases of the diacylglycerol kinase family. This “regulation loop” is reminiscent of the “activation loop” that controls AGC protein kinases, being in a similar distance from the critical ATP binding site determinants in the primary sequence.
| Item Type: | Article |
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| Additional Information: | Archiving not formally supported by this publisher |
| Keywords: | phosphorylation; ceramide; kinase; activation loop; glycine-rich loop; LC-MS |
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| Date Deposited: | 13 Oct 2015 13:17 |
| Last Modified: | 13 Oct 2015 13:17 |
| URI: | https://oak.novartis.com/id/eprint/1287 |