Peters, Francis B, Brock, Ansgar, Wang, Jiangyun and Schultz, Peter (2009) Photocleavage of the Polypeptide Backbone by 2-Nitrophenylalanine. Chemistry & Biology, 16 (2). pp. 148-152. ISSN 1879-1301

Abstract

Photocleavage of the polypeptide backbone is potentially a powerful and general method to activate or deactivate functional peptides and proteins with high spatial and temporal resolution. Here we show that 2-nitrophenylalanine is able to photochemically cleave the polypeptide backbone by an unusual cinnoline-forming reaction. This unnatural amino acid was genetically encoded in E. coli, and protein containing 2-nitrophenylalanine was expressed and site-specifically photocleaved.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... http://www.cell.com/chemistry-biology/re...
Keywords:	Chembio
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... http://www.cell.com/chemistry-biology/re...
Date Deposited:	14 Dec 2009 13:49
Last Modified:	31 Jan 2013 00:57
URI:	https://oak.novartis.com/id/eprint/1220