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Methylated DNA immunoprecipitation (MeDIP).

Mohn, Fabio and Weber, Michael and Schuebeler, Dirk and Roloff, Tim-Christoph (2009) Methylated DNA immunoprecipitation (MeDIP). Methods in Molecular Biology (Clifton, N.J.), 507. pp. 55-64. ISSN 1064-3745

Abstract

Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. In brief, genomic DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. The resulting enrichment of methylated DNA in the immunoprecipitated fraction can be determined by PCR to assess the methylation state of individual regions. Alternatively, MeDIP can be combined with large-scale analysis using microarrays as a genome-wide experimental readout. This protocol has been applied to generate comprehensive DNA methylation profiles on a genome-wide scale in mammals and plants, and further to identify abnormally methylated genes in cancer cells.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: MeDIP; methylated DNA; DNA methylation; genome-wide analysis; epigenomics; 5mC antibody; CpG; immunoprecipitation
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Date Deposited: 14 Dec 2009 13:50
Last Modified: 31 Jan 2013 01:01
URI: https://oak.novartis.com/id/eprint/988

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