Das, Moushumi, Semple, Jennifer I, Haemmerli, Anja, Volodkina, Valeriia, Scotton, Janik, Gitchev, Todor, Annan, Ahrmad, Campos, Julie, Statzer, Cyril, Dakhovnik, Alexander, Ewald, Collin Y, Mozziconacci, Julien and Meister, Peter (2024) Condensin I folds the Caenorhabditis elegans genome. Nature genetics, 56 (8). pp. 1737-1749. ISSN 1546-1718

Abstract

The structural maintenance of chromosome (SMC) complexes-cohesin and condensins-are crucial for chromosome separation and compaction during cell division. During the interphase, mammalian cohesins additionally fold the genome into loops and domains. Here we show that, in Caenorhabditis elegans, a species with holocentric chromosomes, condensin I is the primary, long-range loop extruder. The loss of condensin I and its X-specific variant, condensin I, leads to genome-wide decompaction, chromosome mixing and disappearance of X-specific topologically associating domains, while reinforcing fine-scale epigenomic compartments. In addition, condensin I/I inactivation led to the upregulation of X-linked genes and unveiled nuclear bodies grouping together binding sites for the X-targeting loading complex of condensin I. C. elegans condensin I/I thus uniquely organizes holocentric interphase chromosomes, akin to cohesin in mammals, as well as regulates X-chromosome gene expression.

Item Type:	Article
Date Deposited:	28 Sep 2024 00:45
Last Modified:	28 Sep 2024 00:45
URI:	https://oak.novartis.com/id/eprint/55286