Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

Characterisation of a Refined Rat Model of Respiratory Infection with Pseudomonas aeruginosa and the Effect of Ciprofloxacin

Growcott, Ellie and Coulthard, Alex and Amison, Richard and Hardaker, Liz and Saxena, Vishal and Malt, Layla and Jones, Peter and Grevot, Armelle and Poll, Christopher and Osborne, Colin and Banner, Kathy (2011) Characterisation of a Refined Rat Model of Respiratory Infection with Pseudomonas aeruginosa and the Effect of Ciprofloxacin. Journal of Cystic Fibrosis, 10 (3). pp. 166-174. ISSN 1569-1993

Abstract

BACKGROUND:

We sought to characterise a refined rat model of respiratory infection with P. aeruginosa over an acute time course and test the antibiotic ciprofloxacin.

METHODS:

Agar beads were prepared ± SPAN(®)80. Rats were inoculated with sterile agar beads or those containing 10(5) colony forming units (cfu) P. aeruginosa via intra-tracheal dosing. Bacterial load and inflammatory parameters were measured.

RESULTS:

Differing concentrations of SPAN(®) 80 modified median agar bead diameter and reduced particle size distribution. Beads prepared with 0.01% v/v SPAN(®)80 were evaluated in vivo. A stable lung infection up to 7 days post infection was achieved and induced BALF neutrophilia 2 and 5 days post infection. Ciprofloxacin (50mg/kg) significantly attenuated infection without affecting the inflammatory parameters measured.

CONCLUSION:

SPAN(®) 80 can control the particle size and lung distribution of agar beads and P. aeruginosa-embedded beads prepared with 0.01%v/v SPAN(®)80 can induce infection and inflammation over 7 days.

Item Type: Article
Related URLs:
Related URLs:
Date Deposited: 13 Oct 2015 13:15
Last Modified: 13 Oct 2015 13:15
URI: https://oak.novartis.com/id/eprint/4196

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.