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Identification of a Xist silencing domain 1 by Tiling CRISPR

Wang, Yang and Zhong, Yang and Zhou, Yingyao and Tanaseichuk, Olga and Wilkinson, Jeremy and Li, Zhizhong and Zhao, Jing Crystal (2019) Identification of a Xist silencing domain 1 by Tiling CRISPR. Scientific Reports.

Abstract

Despite the essential roles of long noncoding RNAs (lncRNAs) in development and disease, methods determine lncRNA cis-elements are lacking. Here, we developed a screening method named “Tiling CRISPR” to identify lncRNA functional domains. Using this approach, we identified Xist A-Repeats as the silencing domain, an observation in agreement with published work. Mechanistic analysis suggested a novel function for Xist A-repeats in promoting Xist transcription.

Item Type: Article
Keywords: CRISPR, long noncoding RNA, Xist, A-Repeats, transcription
Date Deposited: 12 Mar 2019 00:45
Last Modified: 12 Mar 2019 00:45
URI: https://oak.novartis.com/id/eprint/36934

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