Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution.

Yap, Thai Leong and Chen, Yen Liang and Xu, Ting and Wen, Daying and Vasudevan, Subhash and Lescar, Julien (2007) A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 63 (Pt 2). pp. 78-83. ISSN 1744-3091

Abstract

Dengue virus, a member of the Flaviviridae genus, causes dengue fever, an important emerging disease with several million infections occurring annually for which no effective therapy exists. The viral RNA-dependent RNA polymerase NS5 plays an important role in virus replication and represents an interesting target for the development of specific antiviral compounds. Crystals that diffract to 1.85 A resolution that are suitable for three-dimensional structure determination and thus for a structure-based drug-design program have been obtained using a strategy that included expression screening of naturally occurring serotype variants of the protein, the addition of divalent metal ions and crystal dehydration.

Item Type: Article
Related URLs:
Additional Information: free final full text version available at PubMedCentral; archiving not allowed on institutional repository
Related URLs:
Date Deposited: 14 Dec 2009 14:01
Last Modified: 31 Jan 2013 01:20
URI: https://oak.novartis.com/id/eprint/305

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.