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Internal standard addition in bioanalysis of dried blood spots: results from the EBF DBS-microsampling consortium

Heudi, Olivier and Van Baar, Ben and Rohde, Morten and Wieling, Jaap and Verhaeghe, Tom and Lüdtke, Silke and De Vries, Ronald and White, Steve and Cobb, Zoe and Timmerman, Philip (2013) Internal standard addition in bioanalysis of dried blood spots: results from the EBF DBS-microsampling consortium. Bioanalysis, 5 (17). pp. 2137-2145. ISSN 1757-6180

Abstract

In the framework of wider exploration of the application of Dried Blood Spots (DBS) in bioanalysis, by the DBS consortium of the European Bioanalytical Forum (EBF), one team of five laboratories investigated the merits of the various ways of internal standard addition prior to LC-MS/MS analysis. A set of 22 pharmaceutical compounds with log P in the range of 0 to 10 was selected for this purpose. Assessments were made of precision, recovery, and of the effects of prolonged storage. Assay precision was not significantly different for 3 months aged samples as compared to ‘fresh’ samples stored for 7 – 22 days. Extraction recovery from 3 months aged spots decreased for some of the analytes. In these cases, the most widely employed addition of IS in the extraction solvent does not adequately compensate for recovery. From the overall results, it is clear that there is no ‘one size fits all’ approach to IS addition in DBS bioanalysis. For some assays the procedure of spiking the IS to the extraction solvent looks perfectly acceptable, but for other assays this way of adding the IS will not compensate for changes in extraction recovery from aged dried blood spots.

Item Type: Article
Keywords: Bioanalysis; Blood analysis; Dried blood spots; DBS; EBF; Internal Standard
Date Deposited: 09 May 2016 23:45
Last Modified: 09 May 2016 23:45
URI: https://oak.novartis.com/id/eprint/9903

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