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Genetically timed, activity-sensor and rainbow transsynaptic viral tools.

Boldogkoi, Zsolt, Balint, Kamill, Awatramani, Gautam B, Balya, David, Busskamp, Volker, Viney, Tim James, Lagali, Pamela, Duebel, Jens, Pasti, Emese, Tombacz, Dóra, Tóth, Judit S, Takacs, Irma F, Gross Scherf, Brigitte and Roska, Botond (2009) Genetically timed, activity-sensor and rainbow transsynaptic viral tools. Nature Methods, 6 (2). pp. 127-130. ISSN 1548-7105


We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
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Date Deposited: 14 Dec 2009 13:50
Last Modified: 31 Jan 2013 01:02


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