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Genetically timed, activity-sensor and rainbow transsynaptic viral tools.

Boldogkoi, Zsolt and Balint, Kamill and Awatramani, Gautam B and Balya, David and Busskamp, Volker and Viney, Tim James and Lagali, Pamela and Duebel, Jens and Pasti, Emese and Tombacz, Dóra and Tóth, Judit S and Takacs, Irma F and Gross Scherf, Brigitte and Roska, Botond (2009) Genetically timed, activity-sensor and rainbow transsynaptic viral tools. Nature Methods, 6 (2). pp. 127-130. ISSN 1548-7105

Abstract

We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
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Date Deposited: 14 Dec 2009 13:50
Last Modified: 31 Jan 2013 01:02
URI: https://oak.novartis.com/id/eprint/971

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