Dendritic spine morphology determines membrane-associated protein exchange between dendritic shafts and spine heads.
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Hugel, Sylvain, Abegg, Mathias, de Paola, Vincenzo, Caroni, Pico, Gaehwiler, Beat H and McKinney, R Anne (2009) Dendritic spine morphology determines membrane-associated protein exchange between dendritic shafts and spine heads. Cerebral Cortex, 19 (3). pp. 697-702. ISSN 1460-2199
Abstract
The purpose of this study was to examine whether variability in the shape of dendritic spines affects protein movement within the plasma membrane. Using a combination of confocal microscopy and the fluorescence loss in photobleaching technique in living hippocampal CA1 pyramidal neurons expressing membrane-linked GFP, we observed a clear correlation between spine shape parameters and the diffusion and compartmentalization of membrane-associated proteins. The kinetics of membrane-linked GFP exchange between the dendritic shaft and the spine head compartment were slower in dendritic spines with long necks and/or large heads than in those with short necks and/or small heads. Furthermore, when the spine area was reduced by eliciting epileptiform activity, the kinetics of protein exchange between the spine compartments exhibited a concomitant decrease. As synaptic plasticity is considered to involve the dynamic flux by lateral diffusion of membrane-bound proteins into and out of the synapse, our data suggest that spine shape represents an important parameter in the susceptibility of synapses to undergo plastic change.
| Item Type: | Article |
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| Additional Information: | author can archive post-print (ie final draft post-refereeing); Publisher version cannot be used except for Nucleic Acids Research articles |
| Keywords: | compartmentalization; FLIP; lateral diffusion; photobleaching; plasticity |
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| Date Deposited: | 14 Dec 2009 13:50 |
| Last Modified: | 31 Jan 2013 01:02 |
| URI: | https://oak.novartis.com/id/eprint/961 |