Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

Dendritic spine morphology determines membrane-associated protein exchange between dendritic shafts and spine heads.

Hugel, Sylvain and Abegg, Mathias and de Paola, Vincenzo and Caroni, Pico and Gaehwiler, Beat H and McKinney, R Anne (2009) Dendritic spine morphology determines membrane-associated protein exchange between dendritic shafts and spine heads. Cerebral Cortex, 19 (3). pp. 697-702. ISSN 1460-2199

Abstract

The purpose of this study was to examine whether variability in the shape of dendritic spines affects protein movement within the plasma membrane. Using a combination of confocal microscopy and the fluorescence loss in photobleaching technique in living hippocampal CA1 pyramidal neurons expressing membrane-linked GFP, we observed a clear correlation between spine shape parameters and the diffusion and compartmentalization of membrane-associated proteins. The kinetics of membrane-linked GFP exchange between the dendritic shaft and the spine head compartment were slower in dendritic spines with long necks and/or large heads than in those with short necks and/or small heads. Furthermore, when the spine area was reduced by eliciting epileptiform activity, the kinetics of protein exchange between the spine compartments exhibited a concomitant decrease. As synaptic plasticity is considered to involve the dynamic flux by lateral diffusion of membrane-bound proteins into and out of the synapse, our data suggest that spine shape represents an important parameter in the susceptibility of synapses to undergo plastic change.

Item Type: Article
Related URLs:
Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher version cannot be used except for Nucleic Acids Research articles
Keywords: compartmentalization; FLIP; lateral diffusion; photobleaching; plasticity
Related URLs:
Date Deposited: 14 Dec 2009 13:50
Last Modified: 31 Jan 2013 01:02
URI: https://oak.novartis.com/id/eprint/961

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.