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"Product ion monitoring" assay for prostate-specific antigen in serum using a linear ion-trap.

Kulasingam, Vathany and Smith, Christopher and Batruch, Ihor and Buckler, Alan and Jeffery, Douglas and Diamandis, Eleftherios P (2008) "Product ion monitoring" assay for prostate-specific antigen in serum using a linear ion-trap. Journal of Proteome Research, 7 (2). pp. 640-647. ISSN 1535-3893

Abstract

While numerous strategies exist for biomarker discovery, the bottleneck to product development and routine use at the clinic is in the verification phase of candidate biomarkers. The aim of this study was to establish a robust and high-throughput product ion monitoring (PIM) assay that is potentially capable of rapidly verifying candidates from discovery phase experiments. Using prostate-specific antigen (PSA), a model biomarker, and a routinely used mass spectrometer for discovery platforms, an ion trap (LTQ, Thermo), the utility of this instrument to perform PIM was explored. The proteotypic doubly charged intact peptide LSEPAELTDAVK ( m/ z 637) fragmenting to m/ z 943 (PAELTDAVK) was monitored. A limit of detection of 10 attomoles with a coefficient of variation (CV) of <20% was obtained for a purified recombinant PSA digest. Immunoextraction of endogenous PSA from serum using a monoclonal antibody on a 96-well microtiter plate, followed by PIM on the LTQ, enabled quantification of PSA down to less than 1 ng/mL with a limit of detection of 0.1 ng/mL and CVs < 20%. Mascot searching and ion ratio confirmation further supported the conclusion that the quantified moiety in serum was the PSA peptide. We conclude that this methodology could be adapted quickly and easily to other candidates, thus providing a much needed technology to bridge the gap between discovery and validation platforms.

Item Type: Article
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Additional Information: archiving not formally supported by this publisher
Keywords: biomarkers; multiple reaction monitoring; prostate-specific antigen; quantification; targeted proteomics; verification phase
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Date Deposited: 14 Dec 2009 13:51
Last Modified: 14 Dec 2009 13:51
URI: https://oak.novartis.com/id/eprint/926

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