Grosshans, Bianka and Novick, Peter (2008) Identification and verification of Sro7p as an effector of the Sec4p Rab GTPase. Methods in Enzymology, 438. pp. 95-108. ISSN 0076-6879

Abstract

Effectors are operationally defined as proteins that recognize a specific GTPase preferentially in its GTP-bound conformation. Here we present the use of affinity chromatography to identify potential effectors of Sec4p, the Rab GTPase that controls the final stage of the yeast secretory pathway. We describe the preparation of the Rab protein affinity matrix and the yeast lysate used in the purification. We also describe the methods used to identify and verify one candidate, Sro7p, as a bona fide Sec4p effector. This includes tests of the specificity and efficiency of binding both in vitro and in vivo.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... doi:10.1016/S0076-6879(07)38007-5
Additional Information:	author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... doi:10.1016/S0076-6879(07)38007-5
Date Deposited:	14 Dec 2009 13:52
Last Modified:	14 Dec 2009 13:52
URI:	https://oak.novartis.com/id/eprint/848