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Capillary electrophoresis of sugar acids.

Campa, Cristiana and Baiutti, Edi and Flamigni, Anna (2008) Capillary electrophoresis of sugar acids. Methods in Molecular Biology (Clifton, N.J.), 384. pp. 307-355. ISSN 1064-3745

Abstract

This chapter illustrates the usefulness of capillary electrophoresis (CE) for the analysis of sugar acids, that is, monosaccharides and lower oligosaccharides carrying carboxylate, sulphate or phosphate groups. In order to provide a general description of the main results and challenges in the field, some relevant applications and reviews on CE of such saccharidic compounds are tabulated. Furthermore, some detailed experimental procedures are shown, regarding the CE analysis of sugar acids released upon hydrolysis of acidic polysaccharides and of glycans linked to glycoproteins. In particular, the protocols will deal with the following compounds: (i) unsaturated, underivatized oligosaccharides from lyase-treated alginate; (ii) oligosaccharides derivatized with 4-aminobenzonitrile, arising from chemical hydrolysis of alginate; (iii) sialic acid derivatized with 2-aminoacridone, released from human serum immunoglobulin G.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: Capillary electrophoresis; sugar acids; sugar phosphates; carboxylated sugars; sulphated sugars; glycosaminoglycans oligosaccharides; uronic acids; sialic acids; tabulated review
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Date Deposited: 14 Dec 2009 13:52
Last Modified: 14 Dec 2009 13:52
URI: https://oak.novartis.com/id/eprint/839

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