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Physicochemical characterisation of glycoconjugate vaccines for prevention of meningococcal diseases.

Bardotti, Angela and Averani, Giovanni and Berti, Francesco and Berti, Stefania and Carinci, Valeria and D'Ascenzi, Sandro and Fabbri, Barbara and Giannini, Sara and Giannozzi, Aldo and Magagnoli, Claudia and Proietti, Daniela and Norelli, Francesco and Rappuoli, Rino and Ricci, Stefano and Costantino, Paolo (2008) Physicochemical characterisation of glycoconjugate vaccines for prevention of meningococcal diseases. Vaccine, 26 (18). pp. 2284-2296. ISSN 0264-410X

Abstract

Bacterial capsular polysaccharides covalently linked to an appropriate carrier protein represent the best tool to induce a protective immune response against a wide range of bacterial diseases, such as meningococcal infections. We describe here the physico-chemical characterisation of glycoconjugate molecules designed to prepare a vaccine against Neisseria meningitidis serogroups A, C, W135 and Y. The use of a selective conjugation chemistry resulted in well characterised, reproducible and traceable glycoconjugate that can be consistently manufactured at large scale. A pool of physical and spectroscopic methods was used to establish glycosylation ratio, identity, molecular weight profiles, integrity of carrier protein and sites of glycosylation, assuring effective and consistent lots of vaccines.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: Glycoconjugate vaccines; Physicochemical characterisation; Neisseria meningitidis
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Date Deposited: 14 Dec 2009 13:52
Last Modified: 14 Dec 2009 13:52
URI: https://oak.novartis.com/id/eprint/834

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