Bardotti, Angela, Averani, Giovanni, Berti, Francesco, Berti, Stefania, Carinci, Valeria, D'Ascenzi, Sandro, Fabbri, Barbara, Giannini, Sara, Giannozzi, Aldo, Magagnoli, Claudia, Proietti, Daniela, Norelli, Francesco, Rappuoli, Rino, Ricci, Stefano and Costantino, Paolo (2008) Physicochemical characterisation of glycoconjugate vaccines for prevention of meningococcal diseases. Vaccine, 26 (18). pp. 2284-2296. ISSN 0264-410X

Abstract

Bacterial capsular polysaccharides covalently linked to an appropriate carrier protein represent the best tool to induce a protective immune response against a wide range of bacterial diseases, such as meningococcal infections. We describe here the physico-chemical characterisation of glycoconjugate molecules designed to prepare a vaccine against Neisseria meningitidis serogroups A, C, W135 and Y. The use of a selective conjugation chemistry resulted in well characterised, reproducible and traceable glycoconjugate that can be consistently manufactured at large scale. A pool of physical and spectroscopic methods was used to establish glycosylation ratio, identity, molecular weight profiles, integrity of carrier protein and sites of glycosylation, assuring effective and consistent lots of vaccines.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez...
Additional Information:	author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords:	Glycoconjugate vaccines; Physicochemical characterisation; Neisseria meningitidis
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez...
Date Deposited:	14 Dec 2009 13:52
Last Modified:	14 Dec 2009 13:52
URI:	https://oak.novartis.com/id/eprint/834