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Elongation Factor P is dispensable in Escherichia coli and Pseudomonas aeruginosa

Balibar, Carl and Mcguire, Dorothy and Dean, Charles (2013) Elongation Factor P is dispensable in Escherichia coli and Pseudomonas aeruginosa. Current Microbiology, 284. ISSN 0343-8651

Abstract

Elongation Factor P (EF-P) is a highly conserved ribosomal initiation factor responsible for stimulating formation of the first peptide bond. Its essentiality has been debated and may differ depending on the organism. Here we demonstrate that EF-P is dispensable in Escherichia coli and Pseudomonas aeruginosa under laboratory growth conditions. Although knockouts are viable, growth rates are diminished compared to wildtype strains. Despite this cost in fitness, these mutants are not more susceptible to a wide range of antibiotics; including ribosome targeting antibiotics such as lincomycin, chloramphenicol, and streptomycin, which have previously been shown to disrupt EF-P function in vitro. In Pseudomonas, knockout of efp leads to an upregulation of mexX, a phenotype previously observed with other genetic lesions affecting ribosome function and that can be induced by treatment with antibiotics affecting protein synthesis.

Item Type: Article
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Date Deposited: 13 Oct 2015 13:14
Last Modified: 13 Oct 2015 13:14
URI: https://oak.novartis.com/id/eprint/8306

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