Antigen generation and display in therapeutic antibody drug discovery – a neglected but critical player
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Ebersbach, Hilmar and Geisse, Sabine (2012) Antigen generation and display in therapeutic antibody drug discovery – a neglected but critical player. Biotechnology Journal. ISSN 1860-6768
Abstract
Disease intervention by targeting a critical pathway molecule via a blocking antibody or interference by therapeutic proteins is currently en vogue. The initialization of these efforts requires inevitably the generation of recombinant proteins or cell-based immunogens as antigens or for therapeutic candidate evaluation, but this critical prerequisite is often overlooked. Thus, one could call the antigen molecule the neglected player in antibody drug discovery.
The variety of methods available for making recombinant proteins or recombinant cell lines presenting the target on the cell surface is huge and needs to be addressed in conjunction with biochemical and biophysical quality criteria and the experimental application intended.
This review therefore tries to capture and summarize the most important aspects of antigen generation and display, enabling sensible decision making in order to give rise to highly affine and potent antibodies.
| Item Type: | Article |
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| Keywords: | antigen preparation, expression systems, quality parameters, immunization, phage display |
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| Date Deposited: | 13 Oct 2015 13:14 |
| Last Modified: | 13 Oct 2015 13:14 |
| URI: | https://oak.novartis.com/id/eprint/7973 |