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GPR39 is coupled to TMEM16a in intestinal fibroblast-like cells

Zeng, Fanning and Wind, Nicholas and Mcclengahan, Conor and Verkuijl, Martin and Watson, Robert and Nash, Mark (2012) GPR39 is coupled to TMEM16a in intestinal fibroblast-like cells. PLoS ONE, 7 (10). e47686.

Abstract

GPR39 is a GPCR implicated as a regulator of gastrointestinal motility, although the mechanism remains elusive. Here, we report that GPR39 is expressed by a specific cell population cultured from mouse small intestine muscle layers, which was subsequently identified as fibroblast-like cells (FLCs) that have recently been shown to modulate gut motility. Application of the GPR39 agonist, Zn2+, induced large currents and membrane depolarization in FLCs cultured from wild-type mice, but not Gpr39−/− mice. This Zn2+-induced current could be suppressed by application of a TMEM16A antagonist, CaCCinh-A01, or by silencing Tmem16a expression. These data suggest that GPR39 might modulate gut motility via regulating TMEM16A function in FLCs.

Item Type: Article
Keywords: GPR39, filbroblast-like cells, TMEM16a
Date Deposited: 13 Oct 2015 13:14
Last Modified: 13 Oct 2015 13:14
URI: https://oak.novartis.com/id/eprint/7720

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