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The Use of Cold Plasma Technology to Reduce Carry-Over in Screening Assays

Kent, Toby and Akhlaq, Mohammed and Rosethorne, Elizabeth and Sattikar, Afrah (2012) The Use of Cold Plasma Technology to Reduce Carry-Over in Screening Assays. Journal of Laboratory Automation. ISSN 2211-0682

Abstract

The accurate transfer of biological reagents represents a fundamental step in the drug screening process and the elimination of carry-over is critical for the generation of accurate measurements of biological activity. The introduction of automated liquid robotics into screening laboratories has transformed the drug screening process, enabling accurate and reproducible transfer of liquids to become a high throughput activity, but has also introduced a new challenge for drug discoverers; to establish screening workflows which limit analyte carry-over for the generation of high quality screening data. The widespread use of pipetting tips on automated liquid handlers often necessitates the use of optimized wash protocols for removing contaminants and frequently requires the use and disposal of large quantities of organic solvents. Furthermore, many chemical and biological reagents are recalcitrant to removal from pipetting tips by treatment with organic solvents. The use of cold atmospheric plasma technology provides an alternative approach for removal of contaminants and offers many advantages over traditional decontamination protocols commonly used during biological screening. This report describes the evaluation of a cold plasma tip cleaning system for reducing carry-over in a range of biological screening assays requiring the transfer of low molecular weight compound, nucleic acid and bacterial liquid transfers. The validation of this technology for biological screening assays is presented and the impact of this technology for screening workflows is discussed.

Item Type: Article
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Date Deposited: 13 Oct 2015 13:14
Last Modified: 13 Oct 2015 13:14
URI: https://oak.novartis.com/id/eprint/7327

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