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A second pilus type in Streptococcus pneumoniae is prevalent in emerging serotypes and mediates adhesion to host cells.

Bagnoli, Fabio and Moschioni, Monica and Donati, Claudio and Dimitrovska, Valentina and Ferlenghi, Ilaria and Facciotti, Claudia and Muzzi, Alessandro and Giusti, Fabiola and Emolo, Carla and Sinisi, Antonella and Hilleringmann, Markus and Pansegrau, Werner and Censini, Stefano and Rappuoli, Rino and Covacci, Antonello and Masignani, Vega and Barocchi, Michele A (2008) A second pilus type in Streptococcus pneumoniae is prevalent in emerging serotypes and mediates adhesion to host cells. Journal of Bacteriology, 190 (15). pp. 5480-5492. ISSN 1098-5530

Abstract

Analysis of publicly available genomes of Streptococcus pneumoniae has led to the identification of a new genomic element containing genes typical of gram-positive pilus islets (PIs). Here, we demonstrate that this genomic region, herein referred to as PI-2 (consisting of pitA, sipA, pitB, srtG1, and srtG2) codes for a second functional pilus in pneumococcus. Polymerization of the PI-2 pilus requires the backbone protein PitB as well as the sortase SrtG1 and the signal peptidase-like protein SipA. Presence of PI-2 correlates with the genotype as defined by multilocus sequence typing and clonal complex (CC). The PI-2-positive CCs are associated with serotypes 1, 2, 7F, 19A, and 19F, considered to be emerging serotypes in both industrialized and developing countries. Interestingly, strains belonging to CC271 (where sequence type 271 is the predicted founder of the CC) contain both PI-1 and PI-2, as revealed by genome analyses. In these strains both pili are surface exposed and independently assembled. Furthermore, in vitro experiments provide evidence that the pilus encoded by PI-2 of S. pneumoniae is involved in adherence. Thus, pneumococci encode at least two types of pili that play a role in the initial host cell contact to the respiratory tract and are potential antigens for inclusion in a new generation of pneumococcal vaccines.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); but on personal or university-hosted websites only
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Date Deposited: 14 Dec 2009 13:56
Last Modified: 14 Dec 2009 13:56
URI: https://oak.novartis.com/id/eprint/676

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