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Production of a conjugate vaccine for Salmonella enterica serovar Typhi from Citrobacter Vi.

Micoli, F and Rondini, S and Pisoni, I and Giannelli, C and Di Cioccio, V and Costantino, P and Saul, A and Martin, L B (2012) Production of a conjugate vaccine for Salmonella enterica serovar Typhi from Citrobacter Vi. Vaccine, 30 (5). pp. 853-861. ISSN 1873-2518

Abstract

A conjugate vaccine for Salmonella enterica serovar Typhi was produced by chemically linking Vi, purified from Citrobacter, to the non-toxic mutant diphtheria toxin CRM(197) via an adipic dihydrazide spacer using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide coupling chemistry. The polysaccharide purification process was developed based on Vi precipitation from culture supernatant with cetyl trimethylammonium bromide (CTAB), solubilization of the CTA-polysaccharide salt with ethanol followed by exchange of the CTA(+) counter ion with Na(+). The purified Vi polysaccharide was fully O-acetylated and with high purity. The conjugation process was optimized to obtain a scalable process that has been used for GMP production at pilot scale of vaccine currently in clinical trials.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
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Date Deposited: 13 Oct 2015 13:14
Last Modified: 13 Oct 2015 13:14
URI: https://oak.novartis.com/id/eprint/6708

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