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Noninvasive assessment of gastric emptying by near-infrared fluorescence reflectance imaging in mice: pharmacological validation with tegaserod, cisapride, and clonidine.

Gremlich, Hans-Ulrich and Martinez, Vicente and Kneuer, Rainer and Kinzy, Willy and Weber, Eckhard and Pfannkuche, Hans-Juergen and Rudin, Markus (2004) Noninvasive assessment of gastric emptying by near-infrared fluorescence reflectance imaging in mice: pharmacological validation with tegaserod, cisapride, and clonidine. Molecular Imaging : official journal of the Society for Molecular Imaging, 3 (4). pp. 303-311. ISSN 1535-3508

Abstract

Noninvasive near-infrared fluorescence reflectance imaging (FRI) is an in vivo technique to assess physiological and molecular processes in the intact organism. Here we describe a method to assess gastric emptying in mice. TentaGel beads with covalently bound cyanine dye (Cy5.5) conjugates as fluorescent probe were administered by oral gavage. The amount of intragastric beads/label was derived from the fluorescence signal intensity measured in a region of interest corresponding to the mouse stomach. The FRI signal intensity decreased as a function of time reflecting gastric emptying. In control mice, the gastric half-emptying time was in agreement with literature data. Pharmacological modulation of gastric motility allowed the evaluation of the sensitivity of the FRI-based method. Gastric emptying was either stimulated or inhibited by treatment with the 5-HT(4) receptor agonists tegaserod (Zelnorm) and cisapride or the alpha(2)-receptor agonist clonidine, respectively. Tegaserod and cisapride dose-dependently accelerated gastric emptying. In contrast, clonidine dose-dependently delayed gastric emptying. In conclusion, FRI using fluorescently labeled beads allows the reliable determination of gastric emptying as well as the assessment of pharmacological interventions. The technique thus offers the potential to characterize molecular targets and pathways involved in physiological regulation and pharmacological modulation of gastric emptying.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); 3 months for STM, 6 months for other titles, except Economics journals which are 12 months; Publisher's version/PDF must be used for post-print deposit; Publisher copyright and source must be acknowledged
Keywords: In vivo imaging; fluorescent dyes; near-infrared imaging; fluorescence reflection imaging; gastric emptying; serotonin; tegaserod; cisapride; serotonin receptors; clonidine
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Date Deposited: 14 Dec 2009 13:56
Last Modified: 31 Jan 2013 01:11
URI: https://oak.novartis.com/id/eprint/650

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