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Antigen Presentation for the Generation of Binding Molecules

Ebersbach, Hilmar, Zhang, Chonghui and Proetzel, Gabriele (2012) Antigen Presentation for the Generation of Binding Molecules. In: Methods in Molecular Biology: Antibody Protocols and Methods. Humana Press. ISBN 978-1-58829-092-2


In last decades several new methods have been established to isolate antibodies, fragments, some even using alternative scaffolds from in vivo and in vitro sources. These methods encompass robust techniques including immunization and hybridoma technology or phage display and also more laborious and novel approaches including ribosome display or B-cell immortalization. All methodologies are dependent upon proper antigen presentation for isolation, screening and further characterization of the selected binding molecules. Here, antigens are classes of molecules including soluble or membrane proteins, part or domains thereof (extracellular domains of GPCR’s), peptides, carbohydrates and small molecular weight moieties. Presentation of antigen in a functional state or perhaps even mimicking the intended application is crucial for successful isolation of useful binding molecules. Further, it is also necessary to consider the expression host and any post translational modifications of target proteins. Increasing demand to target more complex antigens for instance, receptors and ion channels is leading to the development of alternative procedures to present these proteins appropriately, for example by the use of virus like particles and DNA immunization. This chapter describes approaches for the preparation of different forms of immunogens, including synthetic peptides, proteins, including cell-based antigens for immunization and in vitro display systems.

Item Type: Book Section
Keywords: Antigen presentation, Protein, Peptide, Protein expression, Immunization, Monoclonal antibody, in vitro display
Date Deposited: 24 Oct 2012 23:45
Last Modified: 24 Oct 2012 23:45


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